Figure 5. Effects of IVIg on the expression of mTOR and AMPK proteins on CD4⁺ T cells isolated from the PBMCs of healthy donors and cultured under Th17-polarizing conditions. (A) Immunoblotting of p-mTOR, mTOR, p-AMPK, AMPK, p-Akt, Akt, p-S6K, p-STAT3(727), p-STAT3(705), and STAT3 in the CD4⁺ T cells pretreated with IVIg (10 mg/ml) and then cultured under Th17-differentiation conditions for 1 h. (B) Stimulation of CD4⁺ T cells under Th17-differentiation conditions, activated phosphorylation of mTOR, Akt, S6K, STAT3 (705), and STAT3 (727) as detected by Western blotting and shown by the ratio of phosphorylated proteins to total proteins.

^*p<0.05; ^**p<0.01; ^***p<0.001.