Figure 4.

The Extent and Duration of the Innate Immune Responses after Intradermal Electroporation of PBS, pDNA, sa-RNA, and Unmodified and m1ψ-Modified mRNA

Five micrograms of each vector coding for EGFP was injected on the flank of heterozygous IFN-β luciferase reporter mice. (A) In vivo bioluminescence was measured starting 4 h after injection. Signal-to-background ratio was calculated by dividing the bioluminescence signal in the ROIs (total flux, photons per second [p/s]) by its background signal at day −1 for each mouse. Means + SD are displayed (n = 6–8). (B) As a measure of the overall IFN-β expression, the AUCs of the curves in (A) were calculated. The horizontal bars represent the means. *p < 0.05; **p < 0.01; ***p < 0.001.