Figure 5.

Lipid Nanoparticle versus Electroporation-Mediated Intradermal Delivery of sa-RNA

(A) Three mice per group were intradermally injected on both flanks with 5 μg of either sa-RNA-LNPs, naked sa-RNA, or naked sa-RNA followed by electroporation. In vivo bioluminescence was measured over 4 weeks, and mean ± SEM of the total flux in the ROIs is displayed. The injection site was chosen as the ROI and did not include the area of the draining lymph nodes. The dotted line represents the background signal, and the shaded area indicates a significant difference between luciferase expression after use of LNPs versus electroporation. (B) AUC (mean ± SEM) of the expression kinetic curves shown in (A) was calculated and displayed to compare total protein expression between the different delivery systems. Individual values are plotted with the mean. (C) In vivo bioluminescence after intradermal injection of 5, 1, or 0.1 μg sa-RNA encapsulated in LNPs. (D) The calculated AUCs of the curves in (C). The shaded area indicates that the luciferase expression after injection of 5 μg sa-RNA-LNPs is significantly higher than 1 μg and 0.1 μg. Mean ± SEM are displayed. The arrows indicate the time points at which the expression resurgences. *p < 0.05.