Figure 6.

Bioluminescence Images of Four Mice after Intradermal Injection of 5 μg sa-RNA Encapsulated in LNPs

(A and B) In vivo bioluminescence images of (A) the untreated contralateral (negative) flanks and (B) the treated ipsilateral (positive) flanks, respectively, 5 h after injection of sa-RNA-LNPs. Next to the bright bioluminescent spot, which is the injection spot, a small bioluminescent spot is visible at the location of the draining subiliac lymph node (B). Twenty-four hours after injection of the sa-RNA-LNPs, the subiliac lymph nodes were excised, and ex vivo bioluminescent images were taken. (C) and (D) show, respectively, the draining subiliac lymph nodes of the untreated contralateral (negative) flanks and treated ipsilateral (positive) flanks that received sa-RNA-LNPs.