Figure 8.

Kinetics of the Type I Interferon Immune Response after Lipid-Nanoparticle-Mediated Delivery of sa-RNA

(A) The extent and kinetics of the IFN-β induction in reporter mice after LNP- and electroporation-mediated intradermal delivery of 5 μg sa-RNA encoding EGFP was measured by in vivo bioluminescence imaging. The in vivo bioluminescence was measured starting 4 h after injection and was further monitored for 2 weeks. Signal-to-background ratios were calculated by dividing the bioluminescence signals in the ROIs (total flux, p/s), with its background signal at day −1 for each mouse. Means ± SD are displayed (n = 8). (B) As a measure of the overall IFN-β expression, the AUCs of the curves in (A) were calculated. Individual values are plotted with the mean. *p < 0.05; ****p < 0.0001.