Core genome SNP phylogeny of 136 ST258 isolates collected at UPMC from 2010 to 2018. Dotted circles indicate the two major clades (clade I, n =71; clade II = 65). Colored ovals show outbreak-associated isolates with color coding as follows: turquoise, bronchoscope; lavender, ERCP; blue, SICU; brown, gastroscope; green, CTICU; red, EDS-HAT contemporary surveillance isolates. Letters and bracket depict subclades and sublineages described in the text. The scale bar represents the number of substitutions per site.