Table 1.
Primer sequences used for real-time polymerase chain reaction analysis
| Gene | Primer sequence (5´-3´) | Product length (bp) | Accession number |
|---|---|---|---|
| Gdf9 | F: CAAACCCAGCAGAAGTCAC | 194 | NM_008110.2 |
| R: AAGAGGCAGAGTTGTTCAGAG | |||
| Bmp15 | F: AAATGGTGAGGCTGGTAA | 148 | NM_009757 |
| R: TGAAGTTGATGGCGGTAA | |||
| Zp3 | F: CTTGTGGATGGTCTATCTGAG | 125 | NM_011776.1 |
| R: GTGATGTAGAGCGTATTTCTG | |||
| Gja4 (Cx37) | F: CGACGAGCAGTCGGATTT | 155 | NM_008120.3 |
| R: AGATGACATGGCCCAGGTAG | |||
| Gja1(Cx43) | F: TAAGTGAAAGAGAGGTGCCCAGA | 200 | NM_010288.3 |
| R: GGTTGTTGAGTGTTACAGCGAAAAG | |||
| Bmp4 | F: GGTCGTTTTATTATGCCAAGTCC | 417 | NM_001316360.1 |
| R: ATGCTGCTGAGGTTGAAGAGG | |||
| Bmp7 | F: CTATGCTGCCTACTACTGTGAG | 103 | NM_007557.3 |
| R: GTTGATGAAGTGAACCAGTGTC | |||
| Trp53 (p53) | F: AACTTACCAGGGCAACTATG | 203 | NM_001127233.1 |
| R: TGTGCTGTGACTTCTTGTAG | |||
| Casp3 | F: AAAGACCATACATGGGAGC | 138 | NM_001284409.1 |
| R: CGAGATGACATTCCAGTGCT | |||
| Bax | F: TTGCTACAGGGTTTCATCCAG | 246 | NM_007527.3 |
| R: CCAGTTGAAGTTGCCATCAG | |||
| Bcl2 | F: GCCTTCTTTGAGTTCGGT | 162 | NM_009741.5 |
| R: ATATAGTTCCACAAAGGCATCC | |||
| Fshr | F: ACGCCATTGAACTGAGATTTG | 134 | NM_013523.3 |
| R:GAACACATCTGCCTCTATTACC | |||
| Lhcgr | F: AAGCACAGTTAGAGAAGCGA | 244 | NM_013582.3 |
| R: GGTCAGGAGAACAAAGAGGA | |||
| Cyp11a1 | F: TCCTTTGAGTCCATCAGCAG | 180 | NM_001346787.1 |
| R: GTCCTTCCAGGTCTTAGTTCT | |||
| Cyp17a1 | F: AGAAGTGCTCGTGAAGAAGG | 201 | NM_007809.3 |
| R: TTGGCTTCCTGACATATCATCT | |||
| Cyp19a1 | F: ATGTCGGTCACTCTGTACTTC | 107 | NM_001348171.1 |
| R: TTTATGTCTCTGTCACCCACAAC | |||
| Gapdh | F: GACTTCAACAGCAACTCCCAC | 125 | NM_001289726.1 |
| R: TCCACCACCCTGTTGCTGTA | |||