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. 2019 Jul 31;21(4):391–400. doi: 10.22074/cellj.2020.6287

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The Cell Journal (Yakhteh) is an open access journal which means the articles are freely available online for any individual author to download and use the providing address. The journal is licensed under a Creative Commons Attribution-Non Commercial 3.0 Unported License which allows the author(s) to hold the copyright without restrictions that is permitting unrestricted use, distribution, and reproduction in any medium provided the original work is properly cited.

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Fig.5 — Illustrations for histomorphometric analysis of gastrocnemius muscles. A. Representative morphometric analysis of gastrocnemius muscle fibers (×848 magnification) stained with Masson’s trichrome stain and determined using the Dino Capture 2.0 software, B. Histogram showed the effect of ischemia and various interventions on fiber diameter, C. Representative H&E stained a transverse section of muscle demonstrating the calculation of the ratio of the nuclei to the number of muscle fibers (×1000 magnification), and D. Histogram showed the percentage of nuclei to muscle fibers. All values are expressed as the mean ± SEM, a-d represent statistically significant differences (P<0.05) among indicated groups. In this study, we used 5 animals in each group. PRP; Platelet-rich plasma, Mix; Chitosan, PRP, and mast cell group, PBS; Phosphate-buffered saline, and H&E; Hematoxylin and eosin.