Figure 4.

Loss of full-length ORF42 impairs the accumulation of viral proteins. The lytic cycle was induced in the KSHV-infected iSLK.RTA cells by addition of doxycycline (1 μg/mL) to the media. Protein was collected four and six days post-induction. Western blotting was used to measure the levels of the indicated early (a) and late (b) viral proteins. Expression is reported normalized to actin and relative to the WT1 line for the blot shown. All blots shown are from the same biological replicate and are representative of at least 3 biological replicates. Table 2 includes the average and standard deviation values for all the replicates. Several proteins were measured on the same blot and normalized to the same actin blot, as indicated by the blot number. For the glycoprotein K8.1, we used the band corresponding to the size predicted from the sequence for quantification (marked by the asterisk). Other bands likely represent glycosylated forms of K8.1.