Table 2.
Quantification of KSHV protein levels. Average expression and standard deviation of levels of KSHV proteins relative to the WT1 line. n ≥ 3 biological replicates—includes data from blots shown in Figure 1C, Figure 2B, Figure 4 and Figure 5.
| Viral Gene: | WT1 | WT2 | ORF42PTC | ORF42PTC + ORF42 | ORF42PTC + Empty | |
|---|---|---|---|---|---|---|
| Latent | ||||||
| LANA | latent | 1.0 | 1.0 ± 0.2 | 0.9 ± 0.2 | 1.0 ± 0.2 | 0.9 ± 0.3 |
| Day 4 | ||||||
| ORF6 | early | 1.0 | 1.3 ± 0.5 | 0.8 ± 0.2 | 1.9 ± 1.3 | 1.2 ± 0.4 |
| ORF59 | early | 1.0 | 1.2 ± 0.3 | 0.7 ± 0.1 | 1.1 ± 0.2 | 0.8 ± 0.2 |
| ORF68 | early | 1.0 | 1.3 ± 0.5 | 1.1 ± 0.9 | 1.6 ± 1.6 | 0.9 ± 0.5 |
| ORF26 | late | 1.0 | 0.9 ± 0.5 | 0.6 ± 0.5 | 1.3 ± 1.0 | 0.7 ± 0.7 |
| K8.1 | late | 1.0 | 1.9 ± 1.0 | 0.5 ± 0.4 | 1.2 ± 0.7 | 0.8 ± 0.5 |
| ORF33 | late | 1.0 | 0.7 ± 0.4 | 0.8 ± 0.8 | 1.3 ± 0.8 | 0.8 ± 0.1 |
| ORF52 | late | 1.0 | 0.9 ± 0.3 | 1.1 ± 0.5 | 2.1 ± 0.6 | 1.5 ± 0.4 |
| Day 6 | ||||||
| ORF6 | early | 1.0 | 0.8 ± 0.6 | 0.6 ± 0.2 | 0.8 ± 0.5 | 0.7 ± 0.5 |
| ORF59 | early | 1.0 | 1.0 ± 0.4 | 0.7 ± 0.4 | 1.2 ± 0.7 | 0.8 ± 0.3 |
| ORF68 | early | 1.0 | 1.2 ± 0.4 | 0.5 ± 0.2 | 0.9 ± 0.4 | 0.6 ± 0.4 |
| ORF26 | late | 1.0 | 1.5 ± 1.0 | 0.3 ± 0.3 | 0.9 ±0.8 | 0.4 ± 0.5 |
| K8.1 | late | 1.0 | 1.8 ± 1.7 | 0.3 ± 0.4 | 1.3 ± 2.4 | 0.4 ± 0.6 |
| ORF33 | late | 1.0 | 0.9 ± 0.3 | 0.4 ± 0.1 | 1.2 ± 0.3 | 0.7 ± 0.3 |
| ORF52 | late | 1.0 | 2.2 ± 2.6 | 2.0 ± 2.3 | 3.3 ± 3.7 | 2.0 ± 2.3 |