formation of E. coli strains on LBWS (blue bars) and M9glu (yellow bars). The specific biofilm formation index (SBF) is calculated as the ratio between the crystal violet absorbance at 570 nm and the culture turbidity at 600 nm, setting a threshold of 1 for biofilm producers (red dashed line). The reported data are means ± standard deviations of at least three independent experiments, each carried out in triplicate. The names of the strains are colored based on their phylogroups: A, cyan; B1, blue; B2, green; C, yellow; D, red; E, grey; and F, pink (hereafter referred to as LBWS and M9glu, respectively).