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FcRn measurement in human tissues. (A) Correlation of peak area ratio from measured FcRn peptide (Light, L) to SIL internal standard (Heavy, H) of human tissue samples between quantification (GDD) and confirmatory (NGL) peptides, with a high Pearson r score of 0.95. (B) Quantification range of hFcRn in tissue was 0.89–226.35 pmol/g tissue using the peak area ratio of the GDD peptide. Shown is the average of a duplicate calibration curve.
