Figure 1.

Folding and surface expression of hybrid H constructs. (A) Diagram of measles virus H glycoproteins. Created with BioRender.com. H hybrid proteins were constructed by swapping the globular head of the MeV-H or CDV-H among each other, using residue 160 as the delineating point. All hybrids were additionally tagged with a hexahistidine peptide (6 × HIS). Glycans present in the H constructs are indicated and are depicted as black if known to be used, or as gray otherwise. (B) Western blot analysis of hybrid constructs. MelJu-So cells were transfected with the appropriate plasmid, and cell lysates were deglycosylated with PNGase F. Samples were then analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotted with the indicated antibodies. (C) FACS analysis results for cell surface expression. H expression on transiently transfected Expi293F or MelJu-So cells was analyzed through flow cytometry with anti-6 × His antibody. Color coding indicates geometric mean. CDV indicates canine distemper virus; CDV-H, canine distemper virus–H glycoprotein; FACS, fluorescent-activated cell sorting; 6 × H, 6 × HIS; MeV, measles virus; MeV-H, measles virus–H glycoprotein; MeV-H_C, measles virus–H glycoprotein anti–cytoplasmic tail; MeV-H_GH, measles virus–H glycoprotein anti–globular head. (Used with permission of Mayo Foundation for Medical Education and Research).