Figure 6.

Spheroid invasion assay of HCC827 cells treated with vehicle (PBS1x) or Zol 1 µM, 3 µM and 10 µM for 7 and 12 days in 1% serum conditions. (a) Representative brightfield images of HCC827 spheroid invasion/growth within the invasion matrix from vehicle or Zol-treated conditions. (b) Histograms for invasion/growth in the matrix showing the mean of three independent experiments ± SD. Each condition (drug-treated cells or vehicle-treated cells) on each day was normalized to day 0 and then to vehicle. Close to be significantly different from control # p ≥ 0.05.