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. 2019 Aug 3;11(8):391. doi: 10.3390/pharmaceutics11080391

Figure 6.

Figure 6

Naringenin (Nar) and hesperetin (Hesp), incorporated into lipid nanoemulsions, decrease pro-inflammatory markers in TNF-α activated EA.hy926 cells. (A) Quantitative RT-PCR of MCP-1 mRNA expression in TNF-α activated EA.hy926 cells treated with V-Nar/LNs and V-Hesp-LNs as compared with Nar/LNs, Hesp/LNs and free Nar and Hesp; (B) immunoblotting experiments of total cell lysates of TNF-α activated EA.hy926 cells treated as above and probed with anti-MCP1 antibody; (C) immunoblotting of nuclear extracts probed for the p65 subunit, relative to the expression of TATA-binding protein (TBP). *, **, *** depict comparisons between the groups; * represent p < 0.05; ** p < 0.01; *** p < 0.001.