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Analysis of D2LR interaction with MOR using NanoBiT®. HEK 293T cells were transfected with SmBiT and LgBiT-tagged receptor constructs as indicated above. The luminescent signal in cells cotransfected with D2LR fused to SmBiT and MOR fused to LgBiT was significantly higher (~3.5 fold) than the signal of the negative control, cells coexpressing Halotag-SmBiT and MOR-LgBiT. Three independent experiments were performed, and the results were expressed as mean ± SD (** p < 0.01).
