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. 2019 Aug 20;10(8):551. doi: 10.3390/mi10080551

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A,B) Simulation of the food dye concentration gradient: the concentration gradient of the dye within the transversal channel is linear only after 192 h, due to the low value of the diffusion constant; (C,D) simulation of the interleukin-8 (IL-8) concentration gradient: unlike the food dye, the concentration gradient of the interleukins becomes linear after almost 60 min; (E) the concentration values were calculated according to the pixel intensity of images taken in time-lapse along the transversal channel and are therefore expressed in arbitrary units, we report the pixel intensity as a function of the distance 8 h after having filled the device with the blue dye; (F) gradients of concentration of the food dye as a function of the channel position at different times; (G) simulated trend of the concentration gradient within the transverse channel, values expressed in mol/m³; (H) real trend of the concentration gradient.