Table 3.
Analytical sensitivity of Duo CHIKV RT-qPCR, Pastorino and Panning assays.
| West African | Duo | Pastorino | Panning | ||||
| Dilution | Viral RNA copies/µL | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) |
| E-6 | 603 | 6/6 | 32.1 (0.1) | 6/6 | 32.6 (0.2) | 6/6 | 31.8 (0.2) |
| 2E-7 | 106 | 6/6 | 34.3 (0.25) | 6/6 | 35.3 (0.2) | 6/6 | 34.2 (0.1) |
| 4E-8 | 24 | 6/6 | 36.5 (0.43) | 6/6 | 37.3 (0.2) | 6/6 | 36.4 (0.4) |
| 8E-9 | 6 | 5/6 | 38.3 (0.36) | 1/6 | 39.2 | 4/6 | 38.9 (0.2) |
| 1.6E-9 | - | 0/6 | - | 0/6 | - | 0/6 | - |
| 3.2E-10 | - | 0/6 | - | 0/6 | - | 0/6 | - |
| Asian | Duo | Pastorino | Panning | ||||
| Dilution | Viral RNA copies/µL | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) |
| E-6 | 547 | 6/6 | 32.2 (0.2) | 6/6 | 32.3 (0.1) | 6/6 | 31.8 (0.2) |
| 2E-7 | 136 | 6/6 | 34.3 (0.3) | 6/6 | 34.8 (0.3) | 6/6 | 33.7 (0.1) |
| 4E-8 | 32 | 6/6 | 36.1 (0.2) | 6/6 | 37.8 (1.1) | 6/6 | 36.5 (0.8) |
| 8E-9 | 8 | 6/6 | 38 (0.5) | 4/6 | 38.5 (1) | 2/6 | 38.6 (0.9) |
| 1.6E-9 | 2 | 0/6 | - | 3/6 | 39.5 (0.3) | 0/6 | - |
| 3.2E-10 | - | 0/6 | - | 0/6 | - | 0/6 | - |
| ECSA1 | Duo | Pastorino | Panning | ||||
| Dilution | Viral RNA copies/µL | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) |
| E-6 | 146 | 6/6 | 34. 5 (0.2) | 6/6 | 35 (0.2) | 6/6 | 34.6 (0.5) |
| 2E-7 | 39 | 6/6 | 36.4 (0.4) | 6/6 | 37.5 (0.3) | 6/6 | 36.8 (0.1) |
| 4E-8 | 11 | 4/6 | 37.8 (0.6) | 4/6 | 37.6 (0.5) | 1/6 | 39.1 |
| 8E-9 | 5 | 1/6 | 39.2 | 1/6 | 38.7 | 0/6 | - |
| 1.6E-9 | - | 0/6 | - | 0/6 | - | 0/6 | - |
| 3.2E-10 | - | 0/6 | - | 0/6 | - | 0/6 | - |
| ECSA2 | Duo | Pastorino | Panning | ||||
| Dilution | Viral RNA copies/µL | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) |
| E-6 | 859 | 6/6 | 31.9 (0.2) | 6/6 | 31.2 (0.2) | 6/6 | 31.4 (0.1) |
| 2E-7 | 172 | 6/6 | 34.2 (0.1) | 6/6 | 33.8 (0.5) | 6/6 | 33.9 (0.3) |
| 4E-8 | 44 | 6/6 | 36.2 (0.2) | 5*/5 | 36.8 (0.7) | 6/6 | 36.4 (0.8) |
| 8E-9 | 13 | 6/6 | 38 (0.3) | 1/6 | 37.4 | 4/6 | 38.4 (0.6) |
| 1.6E-9 | 6 | 2/6 | 39.1 (0.03) | 0/6 | - | 0/6 | - |
| 3.2E-10 | - | 0/6 | - | 0/6 | - | 0/6 | - |
| ECSA3 | Duo | Pastorino | Panning | ||||
| Dilution | Viral RNA copies/µL | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) | Positive/tested | Ct, Mean (SD) |
| E-6 | 875 | 6/6 | 31.4 (0.1) | 6/6 | 29.7 (0.1) | 5*/5 | 31.7 (0.2) |
| 2E-7 | 204 | 6/6 | 33.6 (0.2) | 6/6 | 32.1 (0.2) | 6/6 | 34 (0.3) |
| 4E-8 | 41 | 6/6 | 36.1 (0.2) | 6/6 | 34.8 (0.5) | 6/6 | 35.9 (0.4) |
| 8E-9 | 13 | 5/6 | 38 (0.9) | 5/6 | 37.1 (0.7) | 5/6 | 38.3 (0.8) |
| 1.6E-9 | 5 | 1/6 | 39.5 | 2/6 | 39.2 (0.03) | 1/6 | 39,3 |
| 3.2E-10 | - | 0/6 | - | 0/6 | - | 0/6 | - |