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. 2019 Aug 12;11(8):408. doi: 10.3390/pharmaceutics11080408

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Effects of Ff35 on ChoKα1 activity assayed in purified ChoKα1 exposed to different Ff35 concentrations. The results are expressed as percentage of enzymatic inhibition. (B) Choline uptake assay in HepG2 cells. Choline uptake was determined in cells treated for 10 min, 24 h, and 48 h soluble Ff35 or Ff35-BMNPs. Results are normalized to their respective control. These experiments were performed three times in triplicates. * p < 0.05, ** p < 0.001 compared to their respective controls.