Figure 6.

Cytokine concentrations produced by ovalbumin (OVA)-stimulated splenocytes corresponded with observed histone acetylation. (A) IL-5, (B) IL-13, (C), IFNγ, (D) IL-10 and (E) IL-17 concentrations measured in supernatant after ex vivo stimulation of splenocytes with OVA for four days (37 °C, 5% CO₂). Data are presented as box-and-whisker Tukey plot (in which outliers are shown as separately plotted points) for IL-5 and IL-13 concentrations and as mean ± standard error of the mean for IFNγ, IL-10 and IL-17 concentrations after subtracting baseline cytokine levels, n = 8/group. * p < 0.05 as analyzed with a Mann-Whitney test (A,B) or an unpaired two-tailed Student’s t-test (C–E). OVA, ovalbumin; raw, raw cow’s milk; shop, shop milk.