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. 2019 Aug 9;11(8):467. doi: 10.3390/toxins11080467

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of aflatoxin biosynthesis gene groups on the production of total aflatoxin by Aspergillus flavus and Aspergillus minisclerotigenes. Gene combinations (A, 000000001, n = 3; B, 000110011, n = 3; C, 001110001, n = 2; D, 001111011, n = 2; E, 011111111, n = 5; and F, 111111111, n = 48). Genes ordered as follows: aflD (aflD-1/aflD-2rev primers), aflD (Nor1-F and Nor-R primers), aflM, aflO, aflP, aflQ (AflQ-1/AflQ-2rev primers), aflQ (Ord1-gF/ Ord1-gR primers), aflR and aflS). Appearance of codes 1 or 0 denotes the gene that is present or absent, respectively in the concatenation.