Table 1.
Cell culture variants of HPA-v.
| I Culture Stage. Proliferation of PAds (5 Days) |
II Culture Stage Differentiation (12 Days) |
III Culture Stage Maturation of Ads (6 Days) |
|---|---|---|
| N | - | - |
| H | - | - |
| N | N | - |
| N | H | - |
| H | N | - |
| H | H | - |
| N | N | N |
| N | N | H |
| N | H | N |
| H | N | N |
| H | H | H |
| H | N | H |
| H | H | N |
| N | H | H |
N: normoglycemic culture conditions (basal medium containing approximately 5.5mM of glucose).H: hyperglycemic culture conditions (medium-supplemented to obtain 30mMd-(+)-glucose concentration).pAds: preadipocytes. Ads: adipocytes. “-”: cells not cultured in a particular step.