Table 1.
Primers used for partial cDNA cloning, RACE reaction, qRT-PCR.
| Primer Name | Primer Sequence (5′-3′) | Application |
|---|---|---|
| Longer | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | Universal primer |
| Shorter | CTAATACGACTCACTATAGGGC | |
| 4CL-5′outer | GGCATTCGCTCTCATCCGCCTCAGTCAG |
4CL RACE reaction |
| 4CL-5′inner | GCTCCACCGGACATGATCAAGC | |
| 4CL-3′outer | ATAGCCCACCTGAAGGTTGCCTCCCGATT | |
| 4CL-3′inner | CTATTCCCTCAATTCGGTGCTC | |
| CCoAOMT-5′outer | GTATTGTCATAGCCGATCACTCCCCCAA | CCoAOMT RACE reaction |
| CCoAOMT-5′inner | CAATTTTGTGGGCAACACCTG | |
| CCoAOMT-3′outer | GCCCTGCCTGATGATGGAAAGATCCTAG | |
| CCoAOMT-3′inner | GATCGGCTATGACAATACTCTG | |
| 4CL-ALL-F | GGGTAAAGATCAATTACTGCTTC | Amplification of the conserved region |
| 4CL-ALL-R | CAAATTTATGTGTGCTGCGAGT | |
| CCoAOMT-ALL-F | GTTTCATTCCTCCAATCCAGT | |
| CCoAOMT-ALL-R | GGGCTGTTCTTAAATCACTCC | |
| RT-4CL-F | CTCCTTTGTGCGCTCCGAGT | RT-PCR |
| RT-4CL-R | GCTCCACCGGACATGATCAAGC | |
| RT-CCoAOMT-F | GCTCATCAATGCCAAGAACACCA | |
| RT-CCoAOMT-R | TCAATTTTGTGGGCAACACCT | |
| CE-4CL-F | ATGGCTTGTGTCGCACCTCT | Enzyme cutting |
| CE-4CL-R | TTAGGCTGCTGCAAGTCTGGC | |
| CE-CCoAOMT-F | ATGGCAACTGCAGAGGCTTC | |
| CE-CCoAOMT-R | AATAACTCTTCTGCAGAGAGTGATGC |