Figure 3.

Hole board behavior in Cyfip1^N/- and Cyfip1^J/- mice. (A): Cartoon and dimensions of the hole board test. The holes (2 × 4) were 2.54 cm in diameter and were spaced center-to-center 10.16 cm apart. Head dips were detected via beam breaks (for additional details, see Supplementary Material). (B): In examining premorbid compulsive-like behavior in the hole board test, for mice on the Cyfip1,2^N/N background, Cyfip1^N/- mice did not differ from wild-type Cyfip1^N/N mice [t(102) = 0.29; P = 0.78]. (C): For mice on the Cyfip1,2^J/J background, Cyfip1^J/- mice showed a significantly greater number of head dips than Cyfip1^J/J mice [t(136) = 2.2; *P = 0.03]. Note that the significant increase in the number of head dips in Cyfip1^J/- mice does not survive the cut-off for significance if one corrects for all 14 phenotypes in the five-day behavioral battery (P < 0.0036; 12 phenotypes in Supplementary Table 2 plus two marble burying phenotypes in Fig. 2).