Figure 2.

Analysis of Pluripotency Marker Expression in iPSCs Generated from RECs

(A) Representative immunofluorescence microscopy images of iPSCs (passage 4) stained with LIN28-, NANOG-, OCT4-, SOX2-, SSEA-4-, and TRA-1-60-specific antibodies. (B) Flow cytometry analysis of iPSCs (passages 5–7) stained with antibodies specific for NANOG and TRA-1-60. Results are shown as mean + SD (n = 3). (C) Expression analysis of SOX2, OCT4, LIN28, NANOG, and E-cadherin transcripts using qRT-PCR. mRNA levels were normalized to GAPDH mRNA levels, and the results are presented relative to the expression levels in RECs. Results are shown as mean + SEM (n = 4). Statistical differences were determined using paired t test (**p < 0.01; ***p < 0.001, ****p<0.0001).