Figure 4.
In Vivo Analysis of Teratoma Formation by Application of iPSCs onto CAM
(A) Schematic representation of the implementation of CAM assay for the analysis of teratoma formation. 2 × 106 iPSCs (passages 8–11) were applied into the inner area of a silicone ring (Ø 8 mm), which was placed onto the CAM on day 7 of incubation. After 10 days of incubation, teratomas (indicated with an arrow) were excised, and histological analyses were performed. (B) A representative microscopic image of H&E-stained teratoma sections showing iPSC-derived tissues of all three germ layers: mesoderm (i, bone-like tissue; ii, connective tissue), endoderm (i, gut-like epithelium; ii, gland-like epithelium), and ectoderm (i, primitive neural rosettes; ii, squamous epithelium).