Supplemental Figure S2.
Pathologic changes in Atg7Δhep-ERT2 mice after alcohol treatment in a chronic-plus-binge model. Mice were acclimated with liquid diet for 5 days. Atg7F/F and Atg7Δhep-ERT2 mice were injected with tamoxifen on days 3 and 4 during acclimation. After acclimation, mice were randomly divided into two groups and given a liquid diet with alcohol (EtOH-fed) or with maltose dextrin (Pair-fed) for 10 days, followed by a single gavage of alcohol (5 g/kg) or isocaloric maltose dextrin. Mice were analyzed 9 hours later. A: Hepatic total cholesterol (TCHO) levels as measured in whole liver or normalized by the liver weight. B–E: Mouse livers were examined histologically by hematoxylin and eosin (H&E) staining (B), immunostaining for F4/80 and quantification (C and D), and trichrome staining (E). Data are expressed as means ± SEM. n = 4–11 (A); n = 4 (B–E). ∗P < 0.05 versus nontreated F/F control; ‡P < 0.05 versus treated F/F control.