Figure 5.

In-vivo tumor inhibitory effect of AC. (A) Photographs of human colon cancer cells, DLD-1 (1 × 10⁶ cells/injection, subcutaneous) were injected into NDO/SCD mice for establishing tumor xenograft model. When tumor size became palpable, mice were separated into four groups: Vehicle control, AC (50 mg/Kg), 5-FU (10 mg/kg), and combination AC + 5-FU (30 mg/kg 5-FU + 10 mg/kg AC). (B) Evaluation of tumor suppressive effect, synergistic effect of AC + 5-FU significantly decreases the size of tumor in the combination treatment group followed by AC and 5-FU individual treatment. (C) Averaged body weight of the animals, there is no negative impact of treatment on the body weight of animals, except for the 5-FU group. * p < 0.05, ** p < 0.01, and *** p < 0.001.