Table 5.
Ultrahigh-Performance Liquid Chromatographic Gradient Profiles and MS Parameters.
| Gradient Profile and Flow Rate of UHPLC | |||
| Total Time (min) | Flow Rate (μL/min) | A (%) | B (%) |
| 0 | 300 | 95 | 5 |
| 12 | 300 | 70 | 30 |
| 20 | 300 | 10 | 90 |
| 20.1 | 300 | 95 | 5 |
| 25 | 300 | 95 | 5 |
| Q-Exactive Parameters | |||
| sheath gas flow rate | 35 | ||
| auxiliary gas flow rate | 10 | ||
| sweep gas flow rate | 0 | ||
| corona current (μA) | 10 | ||
| capillary temperature (°C) | 300 | ||
| s-lens level | 55.0 | ||
| heater temperature (°C) | 300 | ||
| scan range (m/z) | 150–600 | ||
| resolution (± Full scan) | 70,000 | ||
| resolution (dd-MS2) | 17,500 | ||
| NCE | 15 | ||