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. 2019 Aug 2;11(8):709. doi: 10.3390/v11080709

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Viral growth kinetics in MDCK cell. MDCK cells were infected with rgPR8-H9 166N (A), rgPR8-H9 166D (A), XZ299 (B), and m3F2 (B) at an MOI of 0.001, and the tissue culture supernatants were collected at 12, 24, 36, 48, 60, and 72 hpi for the virus titers by TCID₅₀ assays.