Figure 8.

PSMP could also promote macrophage polarization and monocyte adhesion. (A) THP-1 cells were incubated with 10 nM PSMP or CCL2 for 0, 24, 48, or 72 h, and then E-cadherin and β-actin expression levels were measured by Western blotting. (B) after THP-1 cells were incubated with 0, 0.1, 1, or 10 μg/ml PSMP for 4 or 6 h, CCR2 expression on THP-1 membrane was detected by flow cytometry. (C) CCL2 secreted into the medium was measured by ELISA at different time points, at day 3, day 7, and day 9, during which monocytes were induced to differentiate into M2 macrophages with or without incubation with PSMP. (D,E) The expression of several cytokines, including IL1β, IL6, IL10, TNF-α, and TGF-β, were detected by ELISA in the culture medium from M1/M2 macrophages.