Figure 1.

Measles virus (MeV) replication is sensitive to bone marrow stromal antigen 2 (BST2). 293-hSLAMs, transfected with the indicated pcDNA3.1-based expression construct for 24 h, were infected with MeV-GFP at a multiplicity of infection (MOI) of 2 (A–D) and virus replication assayed periodically using an Incucyte imager for 72 h with GFP as a marker (A). In equivalent experiments mean, virus titres were calculated at 48 h post infection by TCID50 analysis of triplicate biological samples of supernatant and cell-associated virus (B); (left panel, sum of total virus titres and right panel, individual fractions). At equivalent time points infected cells were lysed in RIPA buffer and analyzed using western blot to quantify viral protein production (C) or, separately, the RNA was extracted, and the genome was quantified using strand-specific qPCR (D). Error bars indicate standard deviation of the mean. Statistical significance is as follows: *, p < 0.05.