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. 2019 Jul 30;11(8):692. doi: 10.3390/v11080692

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The MeV haemagglutinin glycoprotein is targeted by BST2. (A) Vero-hSLAM cells, cotransfected with the indicated pcDNA3.1-based expression constructs, and analyzed by immunofluorescence microscopy. Representative micrographs are shown together with merged images to demonstrate colocalization of labeled proteins. (B/C) 293-hSLAMs were transfected with 500 ng of pcDNA3.1-MeV nucleocapsid (N) or H and either pcDNA3.1, or, increasing amounts of pcDNA3.1-BST2 (ranging from 0 to 1.6 μg) and analyzed by western blot. The total mass of transfected DNA remained constant.