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. 2019 Jul 30;11(8):692. doi: 10.3390/v11080692

Figure 4.

Figure 4

The BST2 GPI anchor is required for restriction of MeV replication. (A) The human BST2 protein is a membrane anchored protein composed of various domains (TM, transmembrane; GPI, glycosyl-phosphatidylinositol). (B/D) 293-hSLAMs transfected with pcDNA3.1, BST2, ∆-GPI, or ∆-TM were cotransfected with either 500 ng of pcDNA3.1 (B) or 250 ng each of pcDNA3.1-MeV F and H (D) and analyzed by western blot. The total mass of transfected DNA remained constant. (C) The HEK293T MeV effector cells were cotransfected with the indicated pcDNA3.1-based expression constructs and cell fusion quantified by assaying Renilla luciferase activity 16 h after co-culturing with hSLAMF1-positive target cells. (E) The Vero-hSLAM cells, cotransfected with the indicated pcDNA3.1-based expression constructs, and analyzed by immunofluorescence microscopy. Representative micrographs are shown together with merged images to demonstrate colocalization of labeled proteins. (F) 293-hSLAMs, transfected with the indicated pcDNA3.1-based expression construct for 24 h, were infected with MeV-GFP and total virus replication assayed at 72 h. Error bars indicate standard deviation (F) or error (C) of the mean. Statistical significance is as follows: *, p < 0.05; **, p < 0.005; ****, p < 0.0001.