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. 2019 Jul 26;9(8):1074. doi: 10.3390/nano9081074

Figure 4.

Figure 4

Dose- and time-dependent studies showing down-regulation of IL-6 and IL-12p40 by encapsulated and naked IL-10 in mouse J774 macrophages. Macrophages were stimulated with rMOMP and incubated for over a 72 h period with different concentrations of PLA-PEG-IL-10 (1 to 100 ng/mL) and PLA-PEG-PBS (an equivalent nanoparticle weight). At each time-point, namely 24, 48, and 72 h, cytokines were quantified in cell-free supernatants by specific cytokine ELISAs for IL-6 (A–C) and IL-12 p40 (D–F), respectively. p values were calculated by the use of one-way ANOVA, followed by Turkey’s Post-test using GraphPad Prism 6 Software. Statistical significance was established and p values < 0.05 were considered as statistically significant. *** p < 0.001, and **** p < 0.0001. Each bar represents the mean ± SD of samples run in triplicate. ELISA = enzyme-linked immunosorbent assay, IL-6 = Interleukin-6, IL-10 = Interleukin-10, IL-12 p40 = Interleukin-12 p40, PBS = Phosphate-buffered saline, PLA-PEG = Poly (lactic acid)-Poly (ethylene glycol), rMOMP = recombinant major outer membrane protein.