Figure 3.

Endogenous p8 shows increased anti-cancer activity. (A) To examine whether endogenous expression improves the anti-cancer activity of endogenous p8 expression, we examined its anti-proliferative effects in an MTT assay. (B) Colony formation after endogenous expression of p8 was determined by staining with crystal violet. (C) The anti-migration activity of endogenous p8 expression was determined in a wound healing assay. Wound recovery was analyzed using Image J. (D) Anti-cancer efficacy of endogenous p8 expression was examined under an ImageXpress® Micro Confocal microscope. Cells were stained with the live/dead cell markers Syto9 (Green)/EthD-1 (Red) or with the total cell marker Hoechst (Blue).