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. 2019 Aug 8;7(8):245. doi: 10.3390/microorganisms7080245

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Visualization of two unique characters of dinoflagellate chromatin. (A) Assay for Transposase-Accessible Chromatin (ATAC)-see results (A1) Brightfield image of two Hematodinium sp. cells. (A2) DNA staining of the same cells (A3) ATAC-see results show that in dinoflagellates at least two chromatin states exist with one nucleus highly stained while a second nucleus is not affected. This resembles accessible euchromatin and inaccessible heterochromatin states found in nuclei of other non-dinoflagellate eukaryotes. (A4) Merged image of A2 and A3. (B) DVNP immunolocalization. (B1) Brightfield image of a Hematodinium sp. cell. (B2) DNA staining of the same cell. (B3) DVNP distribution in the nucleus revealed using a polyclonal anti-DVNP antibody. (B4) Merged image of B2 and B3.