Figure 3.

Graphical representation of cloning of the FIV genome and full-length Pr50^Gag-His₆-tag bacterial expression plasmid. (A) Schematic representation of the FIV genome and VP10 FIV full-length Pr50^Gag bacterial expression plasmid showing Gag precursor domains and His₆-tag. (B) FIV full-length Pr50^Gag nucleotide and amino acid sequences with mutations introduced (shown as insets) to lose two XhoI sites for cloning purposes without changing the amino acid sequence. (C) Schematic representation of VP10- FIV full-length Pr50^Gag after cloning Gag sequences in the modified pET28b (+) vector in which Pr50^Gag is expressed from the bacteriophage T7 promoter.