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. 2019 Jul 27;11(8):689. doi: 10.3390/v11080689

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Size exclusion chromatography and western blot analysis of the IMAC-purified recombinant Pr50^Gag-His₆-tag protein. (A) Chromatogram showing a distinct peak (between fractions 27 and 32) of the purified recombinant full-length FIV Pr50^Gag-His₆-tag fusion protein. (B) Coomassie Brilliant Blue-stained SDS-PAGE following electrophoresis of fractions 27–32, which reveals the presence of the purest form of the recombinant full-length FIV Pr50^Gag-His₆-tag fusion protein in fractions 28 and 29. (C) Western blot analyses on the pooled fractions (fractions 28 and 29) of the purified protein using α-His₆ and FIV α-p24 monoclonal antibodies, respectively, using equal amounts of protein.