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. 2019 Jul 30;10(8):578. doi: 10.3390/genes10080578

Table 1.

Nanoplot statistics for the MinIT base-calling output and post MinION Detection Software (MINDS) statistics for the Centrifuge analysis. The average multi-classified read mapped to 2.53 species (Centrifuge multi-classified reads count/Actual MinION reads that Centrifuge multi-classified).

Mean Read Length 2268 bp
Mean read quality 9.6
Medium read length 1344 bb
Median read quality 9.7
Number of reads 173,440 bp
Read length N50 4275 bp
Total bases 393,502,204 bp
Reads above quality cutoffs > Q5 173,440 (100%)
Reads above quality cutoffs > Q7 173,440 (100%)
Reads above quality cutoffs > Q10 68,766 (39.6%)
Reads above quality cutoffs > Q12 1344 (0.8%)
Reads above quality cutoffs > Q12 0 (0%)
Number of reads mapped by Centrifuge 184,795
Unclassified reads by Centrifuge 5611
Centrifuge total reads count 179,184
Centrifuge unique reads count 160,396
Centrifuge multi-classified reads count 18,788
Actual MinION reads that Centrifuge multi-classified 7433