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. 2019 Jul 29;17(8):447. doi: 10.3390/md17080447

Figure 4.

Figure 4

Effect of HMWF on NK cell cytotoxic activity and granzyme B release in cyclophosphamide (CY)-treated mice. Immunosuppression was induced by injection of CY (100 mg/kg body weight (b.w.)/d, i.p.) on day 21. The control (CON) group and CY-treated only (CY only) group was orally administered with vehicles alone from day 1 to day 28, while the HWMF-low treated group (HMWF low), and HMWF- high treated group (HMWF high), were administered HWMF at a dosage of 25 and 75 mg/kg b.w., respectively. The CY only group, HMWF-, and LMWF-treated groups were injected with CY on day 21. Splenocytes were incubated with YAC-1 cells for 4 h. (A) NK cell cytotoxic activity was measured by LDH assay. (B) Granzyme B release was measured by enzyme-linked immunosorbent assay. Values are presented as mean ± standard deviation (n = 6). Different letters indicate significant differences from the CY only group at p < 0.05 by a Duncan test.