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. 2019 Aug 7;10(8):596. doi: 10.3390/genes10080596

Figure 3.

Figure 3

Figure 3

Figure 3

Figure 3

Single and multiplex mutagenesis of SR proteins using the PTG system. sgRNAs were designed to target each SR locus mostly in the first or second exons. The sgRNA target sequence is indicated in bold. The protospacer adjacent motif (PAM) sequence is underlined. Blue boxes indicate exons, and lines represent introns. Multiplex targeting was done for each subgroup of the SR family, in which all loci were targeted simultaneously. The multiplex mutants were named according to their subfamily name preceded by “M” for “multiplex.” (A) Single and multiplex mutants are identified for the SR subfamily; (B) single and multiplex mutants for RSZ and SC subfamilies; (C) the SCL subfamily has six members. Multiplex targeting was done for SCL25/26/28 and SCL30a/30/57. SCL30a and SCL30 are highly conserved; a single sgRNA was used to target both loci; (D) mutagenesis of RS2Z and RS subfamilies. RS2Z36 and RS2Z38 are highly conserved, and a single sgRNA was used to target both loci.