. 2019 Aug 20;7(8):272. doi: 10.3390/microorganisms7080272

Table 4.

Biofilm-forming ability of non-O157 E. coli isolates on polystyrene and number of isolates positive for biofilm forming genes in isolates that were positive for Shiga toxin genes and multi-drug resistant non-STEC.

Sampling Region	Biofilm-Forming Ability				Biofilm-Forming Genes
	Strong Biofilm (OD 0.32)		Weak Biofilm (OD 0.082)		csgA	csgD	crl	fimH	flu	rpoS	ehaA^α	ehaA^β
	22 °C	37 °C	22 °C	37 °C	csgA	csgD	crl	fimH	flu	rpoS	ehaA^α	ehaA^β
Koster dairy n = 16	10	5	6	11	16	16	16	15	9	13	11	9
Vryburg beef n = 6	5	5	1	1	6	6	6	6	4	6	4	1
Rooigrond dairy n = 18	7	3	11	15	18	18	18	16	14	10	9	5
Rooigrond beef n = 24	23	11	1	13	24	24	24	24	22	24	15	5
% Isolates indicating biofilm-forming ability and each biofilm-forming gene	70.3	37.5	29.7	62.5	100	100	100	95.3	76.5	82.8	60.9	31.2

n = number of isolates; csgA: Curli structural subunit, csgA: Curli regulator D, crl: Curli regulator, fimH: Type 1 fimbriae, flu: Antigen 43 autotrasporter protein, rpoS: DNA-binding proteins for regulating csgD, ehaA^α: Eha passenger and ehaA^β: Eha translocation domain.