Fig. 5.

Overexpression of E. coli ABCF Uup suppresses cold sensitivity and ribosome assembly defects caused by loss of translational GTPase BipA. Growth (A) and sucrose gradient polysome analysis (B) of CFT073 wild-type, isogenic ΔbipA and ΔbipAΔuup, as well as CFT073 ΔbipA transformed with low-copy pSC vector expressing either BipA or ABCFs EttA, Uup, YheS and YbiT under control of constitutive promoter P_tet. All experiments were performed in filtered LB at 18 °C, and data are presented as geometric means ± standard deviation (n = 3).