Table 2.
APAP and its metabolites in the plasma, liver, and urine after APAP challenge for 12 h a.
| 4 h | 12 h | |||
|---|---|---|---|---|
| APAP | APAP + EGCG | APAP | APAP + EGCG | |
| Plasma | ||||
| APAP (μg/mL) | 259.4 ± 130.3 | 187.9 ± 18.1 | 303.1 ± 61.0 | 387.5 ± 62.9 |
| APAP–glucuronide (μg/mL) | 101.2 ± 5.2 | 82.2 ± 6.9 * | 59.5 ± 9.3 | 51.4 ± 10.9 |
| APAP–sulfate (μg/mL) | 224.2 ± 102.9 | 163.8 ± 23.9 | 29.1 ± 14.1 | 19.8 ± 8.7 |
| APAP–GSH (μg/mL) | 12.1 ± 9.4 | 11.9 ± 6.1 | 32.5 ± 10.9 | 11.0 ± 9.7 * |
| Liver | ||||
| APAP (μg/g liver) | 115.7 ± 33.1 | 128.9 ± 55.6 | 77.3 ± 31.9 | 95.9 ± 33.0 |
| APAP–glucuronide (μg/g liver) | 618.1 ± 211.7 | 450.2 ± 89.1 | 432.5 ± 181.3 | 520.3 ± 157.8 |
| APAP–sulfate (μg/g liver) | 52.5 ± 15.7 | 69.4 ± 16.1 | 49.3 ± 18.0 | 44.9 ± 14.9 |
| APAP–GSH (μg/g liver) | 438.6 ± 88.2 | 447.8 ± 140.2 | 645.2 ± 230.6 | 344.2 ± 122.2 * |
| APAP–protein adducts (mg/g liver) | 1.8 ± 0.4 | 1.1 ± 0.2 * | 3.5 ± 1.0 | 2.1 ± 0.4 * |
| Urine | ||||
| APAP (mg/12 h) | 3.3 ± 0.7 | 5.4 ± 0.7 * | ||
| APAP–glucuronide (mg/12 h) | 144.5 ± 61.5 | 155.7 ± 14.2 | ||
| APAP–sulfate (mg/12 h) | 209.2 ± 39.1 | 189.6 ± 13.5 | ||
a Results are expressed as the mean ± S.D. of four (for 4 h) or six (for 12 h) rats in each group. APAP, N-acetyl-p-aminophenol. * Significantly different from APAP group, p < 0.05.