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Nuclear magnetic resonance-detected high-pressure unfolding of DEN4-ED3 at 10 °C and 0.5 M guanidinium chloride. (A–C) Examples of {1H-15N} HSQC NMR spectra at different pressures as indicated; (D) overlay of the normalized residue-specific denaturation curves as obtained from the fit of the pressure-dependent sigmoidal decrease of the residue cross-peak intensities in the HSQC spectra with Equation (1).
