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. 2019 Jul 25;9(8):302. doi: 10.3390/biom9080302

Figure 2.

Figure 2

CBD induced G0–G1 cell cycle arrest of SGC-7901 cells. (a) Flow cytometry was used to determine the cell cycle distribution of CBD-treated SGC-7901 cells. (b) Statistical analysis of the cell cycle distribution of SGC-7901 cells treated by CBD. (c) SGC-7901 cells were treated with CBD for 24 h, followed by 24 h of incubation with fresh culture media (FM), and then the cycle distribution was examined by flow cytometry. (d) Statistical analysis of the cell cycle distribution of SGC-7901 cells after 24 h of CBD treatment and 24 h of incubation with fresh culture media (FM). (e) The expression levels of CDK2 and cyclin E protein in CBD-treated SGC-7901 cells were detected by western blotting. (f) Statistical analysis of the levels of CDK2 and cyclin E in CBD-treated SGC-7901 cells. The ratio of protein levels was normalized according to the values of the control. All data are expressed as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 compared to the control.