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. 2019 Jul 25;9(8):302. doi: 10.3390/biom9080302

Figure 5.

Figure 5

Effect of CBD on the expression of apoptosis-related proteins. (a) After 24 h of treatment with CBD, the protein expression levels of caspase-9, cleaved caspase-9, caspase-3, and cleaved caspase-3 in SGC-7901 cells were determined by western blotting. (b) Statistical analysis of protein relative levels of caspase-9, cleaved caspase-9, caspase-3, and cleaved caspase-3 in SGC-7901 cells treated with CBD. The ratio of protein levels was normalized according to the values of the control. (c) After 24 h of treatment with CBD, the levels of cytoplasm cytochrome C and Apaf-1 in SGC-7901 cells were determined by western blotting. (d) Statistical analysis of the levels of cytoplasm cytochrome C and Apaf-1 in CBD-treated SGC-7901 cells. The ratio of protein levels was normalized according to the values of the control. All data are expressed as the mean ± SD of three independent experiments. * p < 0.05, ** p < 0.01 compared to the control.