Table 4.
Relative quantification of identified compounds in sample’s 22 fractions.
| Soaking Water | Soaked Coats | Soaked Cotyledons | Whole Flour (Raw) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Class | Name | Area (%, Area/Total Area) | %, Area/Total Compounds’ Class Area | Area (%, Area/Total Area) | %, Area/Total Compounds’ Class Area | Area (%, Area/Total Area) | %, Area/Total Compounds’ Class Area | Area (%, Area/Total Area) | %, Area/Total Compounds’ Class Area |
| HBA | (1) Protocatechuic acid-4-O-Gl | 1677120 (2.9) | 43.2 | 85308 (0.6) | 20.5 | 451623 (4.8) | 31.2 | 13153 (0.03) | 8.1 |
| (2) Vanillic acid | 67863 (0.1) | 1.7 | 4363 (0.03) | 1.0 | 15603 (0.2) | 1.1 | 29105 (0.1) | 17.9 | |
| (5) Protocatechuic acid | 130651 (0.2) | 3.4 | 137055 (1.0) | 33.0 | 9253 (0.1) | 0.6 | 25185 (0.1) | 15.5 | |
| (12) p-Hydroxybenzoic acid-4-O- Gl | 1841267 (3.2) | 47.4 | 163157 (1.2) | 39.3 | 949047 (10.1) | 65.6 | 23743 (0.1) | 14.6 | |
| (25) p-Hydroxybenzoic acid | 60267 (0.1) | 1.6 | 19416 (0.1) | 4.7 | 13706 (0.1) | 0.9 | 69860 (0.2) | 43.0 | |
| (27) Gentisic acid | 106891 (0.2) | 2.8 | 6345 (0.05) | 1.5 | 7149 (0.1) | 0.5 | 1446 (0.003) | 0.9 | |
| Total compounds’ class area | 3884059 (6.6) | 100.0 | 415644 (3.0) | 100.0 | 1446381 (14.7) | 100.0 | 162493 (0.4) | 100.0 | |
| HCA | (3) p-Coumaroyl aldaric acid 1 | 26590 (0.05) | 0.6 | 4318 (0.03) | 2.2 | 167283 (1.8) | 3.8 | 114749 (0.3) | 1.5 |
| (6) p-Coumaroyl aldaric acid 2 | 139230 (0.2) | 3.3 | 8798 (0.1) | 4.5 | 42992 (0.5) | 1.0 | 73863 (0.2) | 1.0 | |
| (7) Feruloyl aldaric acid 1 | 97092 (0.2) | 2.3 | 7766 (0.1) | 4.0 | 305601 (3.3) | 6.9 | 689964 (1.7) | 9.2 | |
| (8) p-Coumaroyl aldaric acid 3 | 1265546 (2.2) | 30.4 | 22910 (0.2) | 11.7 | 417109 (4.4) | 9.5 | 496423 (1.2) | 6.6 | |
| (9) Sinapoyl aldaric acid 1 | 35954 (0.1) | 0.9 | 1977 (0.01) | 1.0 | 65773 (0.7) | 1.5 | 183550 (0.4) | 2.5 | |
| (10) Feruloyl aldaric acid 2 | 12142 (0.02) | 0.3 | 3078 (0.02) | 1.6 | 137695 (1.5) | 3.1 | 130905 (0.3) | 1.7 | |
| (13) Feruloyl aldaric acid 3 | 66532 (0.1) | 1.6 | 3551 (0.03) | 1.8 | 49572 (0.5) | 1.1 | 139151 (0.3) | 1.9 | |
| (14) p-Coumaroyl aldaric acid 4 | 321109 (0.6) | 7.7 | 5263 (0.04) | 2.7 | 9651 (0.1) | 0.2 | 9516 (0.02) | 0.1 | |
| (15) p-Coumaroyl aldaric acid 5 | 150575 (0.3) | 3.6 | 17330 (0.1) | 8.9 | 396951 (4.2) | 9.0 | 339760 (0.8) | 4.5 | |
| (16) Feruloyl aldaric acid 4 | 139602 (0.2) | 3.3 | 3934 (0.03) | 2.0 | 213762 (2.3) | 4.9 | 125167 (0.3) | 1.7 | |
| (19) Sinapoyl aldaric acid 2 | 49814 (0.1) | 1.2 | 1705 (0.01) | 0.9 | 55150 (0.6) | 1.3 | 14544 (0.03) | 0.2 | |
| (20) Sinapoyl aldaric acid 3 | 172631 (0.3) | 4.1 | 5470 (0.04) | 2.8 | 242307 (2.6) | 5.5 | 677720 (1.6) | 9.0 | |
| (22) Feruloyl aldaric acid 5 | 470732 (0.8) | 11.3 | 44560 (0.3) | 22.8 | 757939 (8.1) | 17.2 | 1381715 (3.3) | 18.4 | |
| (23) Sinapoyl aldaric acid 4 | 171674 (0.3) | 4.1 | 8603 (0.06) | 4.4 | 291186 (3.1) | 6.6 | 747370 (1.8) | 10.0 | |
| (26) p-Coumaroyl aldaric acid 6 | NF | NF | 18685 (0.1) | 9.6 | 433166 (4.4) | 9.0 | 213995 (0.5) | 2.9 | |
| (30) Feruloyl aldaric acid 6 | 730915 (1.3) | 17.5 | 28548 (0.2) | 14.6 | 971927 (10.4) | 22.1 | 1644680 (4.0) | 22.0 | |
| (31) Sinapoyl aldaric acid 5 | 185506 (0.3) | 4.5 | 7616 (0.05) | 3.9 | 273562 (2.9) | 6.2 | 465873 (1.1) | 6.2 | |
| (40) p-Coumaric acid | 89291 (0.2) | 2.1 | 338 (0.002) | 0.2 | NF | NF | 11431 (0.03) | 0.2 | |
| (43) Sinapic acid | 2151 (0.004) | 0.1 | 418 (0.003) | 0.2 | 8055 (0.1) | 0.2 | 6305 (0.02) | 0.1 | |
| (44) Ferulic acid | 40918 (0.1) | 1.0 | 158 (0.001) | 0.1 | NF | NF | 23022 (0.1) | 0.3 | |
| Total compounds’ class area | 4168002 (7.1) | 100.0 | 195028 (1.4) | 100.0 | 4839680 (49.3) | 100.0 | 7489702 (18.0) | 100.0 | |
| Flavanols | (4) (+)-Catechin 3’-O-Gl | 7636898 (13.0) | 33.5 | 1165545 (8.3) | 32.7 | 518148 (5.3) | 86.3 | 3166476 (7.6) | 31.6 |
| (11) Procyanidin B1 | 4199734 (7.2) | 18.4 | 866524 (6.2) | 24.3 | NF | NF | 1885313 (4.5) | 18.8 | |
| (17) (+)-Catechin 7-O-Gl | 1214896 (2.1) | 5.3 | 70095 (0.5) | 2.0 | 10469 (0.1) | 1.7 | 172724 (0.4) | 1.7 | |
| (18) Procyanidin B2 | 926982 (1.6) | 4.1 | 197566 (1.4) | 5.5 | 2303 (0.02) | 0.4 | 468641 (1.1) | 4.7 | |
| (21) Procyanidin C1 | 1216935 (2.1) | 5.3 | 546543 (3.9) | 15.3 | 2018 (0.02) | 0.3 | 265008 (0.6) | 2.6 | |
| (24) (+)-Catechin | 5800883 (9.9) | 25.4 | 507760 (3.6) | 14.2 | 60537 (0.6) | 10.1 | 3025744 (7.3) | 30.2 | |
| (28) Procyanidin B3 | 205083 (0.4) | 0.9 | 21266 (0.2) | 0.6 | NF | NF | 38912 (0.1) | 0.4 | |
| (32) Procyanidin B4 | 60776 (0.1) | 0.3 | 8658 (0.1) | 0.2 | NF | NF | 28180 (0.1) | 0.3 | |
| (33) (-)-Epicatechin | 559522 (1.0) | 2.5 | 51252 (0.4) | 1.4 | 4432 (0.05) | 0.7 | 353630 (0.8) | 3.5 | |
| (35) Procyanidin C2 | 185749 (0.3) | 0.8 | 25218 (0.2) | 0.7 | 171 (0.002) | 0.0 | 85610 (0.2) | 0.9 | |
| (36) Procyanidin B5 | 820786 (1.4) | 3.6 | 104129 (0.7) | 2.9 | 2311 (0.02) | 0.4 | 522375 (1.3) | 5.2 | |
| Total compounds’ class area | 22828243 (39.0) | 100.0 | 3564555 (25.5) | 100.0 | 600389 (6.1) | 100.0 | 10012612 (24.0) | 100.0 | |
| Flavanones | (29) Eriodictyol-hexoside 1 | 98920 (0.2) | 1.7 | 4350 (0.03) | 1.7 | 4011 (0.04) | 1.3 | 197075 (0.5) | 2.7 |
| (34) Eriodictyol-hexoside 2 | 5166817 (8.8) | 86.4 | 178852 (1.3) | 70.6 | 272049 (2.8) | 90.9 | 5800730 (13.9) | 80.6 | |
| (37) Eriodictyol-hexoside 3 | 252875 (0.4) | 4.2 | 25333 (0.2) | 10.0 | 12016 (0.1) | 4.0 | 844769 (2.0) | 11.7 | |
| (38) Eriodictyol-hexoside 4 | 177730 (0.3) | 3.0 | 5960 (0.04) | 2.4 | 804 (0.01) | 0.3 | 165914 (0.4) | 2.3 | |
| (42) Naringenin-7-Gl | 10713 (0.02) | 0.2 | 585 (0.004) | 0.2 | 409 (0.004) | 0.1 | 5652 (0.01) | 0.1 | |
| (50) Eriodictyol | 254008 (0.4) | 4.2 | 28839 (0.2) | 11.4 | 1324 (0.01) | 0.4 | 161919 (0.4) | 2.3 | |
| (53) Naringenin | 15799 (0.03) | 0.3 | 9365 (0.1) | 3.7 | 8739 (0.1) | 2.9 | 17103 (0.04) | 0.2 | |
| Total compounds’ class area | 5976862 (10.2) | 100.0 | 253284 (1.8) | 100.0 | 299352 (3.0) | 100.0 | 7193164 (17.3) | 100.0 | |
| Flavonols | (39) Luteolin 3,7-di-O-Gl or Kaempferol-3′,7-dihexoside | 309103 (0.5) | 1.4 | 20371 (0.1) | 0.2 | 2187 (0.02) | 0.1 | 535830 (1.3) | 3.2 |
| (41) Rutin | 208461 (0.4) | 1.0 | 20628 (0.1) | 0.2 | 14381 (0.1) | 0.6 | 86221 (0.2) | 0.5 | |
| (45) Kaempferol-3-O-xylosyl-Gl | 2737477 (4.7) | 12.7 | NF | NF | NF | NF | 2217478 (5.3) | 13.2 | |
| (46) Quercetin-3-O-Gl | 1233782 (2.1) | 5.7 | 8850 (0.1) | 0.1 | NF | NF | 1485967 (3.6) | 8.9 | |
| (47) Quercetin-3-(6-O-acetyl-Gl) | 62179 (0.1) | 0.3 | 37 (0.003) | 0.0 | NF | NF | 63797 (0.2) | 0.4 | |
| (48) Kaempferol-3-O-Gl | 11475034 (19.6) | 53.2 | 6344589 (45.4) | 66.5 | 438101 (4.5) | 16.8 | 7437720 (17.9) | 44.3 | |
| (49) Kaempferol-3-O-acetyl-Gl | 5393293 (9.2) | 25.0 | 1228219 (8.8) | 12.9 | 1942204 (19.8) | 74.7 | 4250372 (10.2) | 25.3 | |
| (52) Quercetin | 8100 (0.01) | 0.0 | 12113 (0.1) | 0.1 | 10403 (0.1) | 0.4 | 17618 (0.04) | 0.1 | |
| Flavonols | (55) Kaempferol | 145232 (0.3) | 0.7 | 1903123 (13.6) | 20.0 | 194088 (2.0) | 7.5 | 682007 (1.6) | 4.1 |
| Total compounds’ class area | 21572661 (36.9) | 100.0 | 9537931 (68.2) | 100.0 | 2601364 (27.7) | 100.0 | 16777009 (40.3) | 100.0 | |
| Isoflavones | (51) Daidzein | 2243 (0.004) | 25.2 | 2434 (0.02) | 23.0 | 9867 (0.1) | 34.6 | 538 (0.001) | 100.0 |
| (54) Genistein | 6664 (0.01) | 74.8 | 8165 (0.1) | 77.0 | 18661 (0.2) | 65.4 | NF | NF | |
| Total compounds’ class area | 8907 (0.02) | 100.0 | 10599 (0.1) | 100.0 | 28528 (0.3) | 100.0 | 538 (0.001) | 100.0 | |
| Total area | 58438734 (100.0) | 13977041 (100.0) | 9815694 (100.0) | 41635518 (100.0) | |||||
HBA—Hydroxybenzoic Acid; HCA—Hydroxycinnamic Acid; Gl—Glucoside; NF—Not Found; the underlined percentage(s) indicate, per compounds’ class the most abundant compound(s) in each fraction.