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. 2019 Aug 7;11(8):728. doi: 10.3390/v11080728

Figure 2.

Figure 2

Figure 2

miR-125b binds the Sema4D 3′UTR. (A) TargetScan predicted the seed regions of the Sema4D 3′-UTR that bind miR-125b. (B) Luciferase assays were performed after co-transfecting reporter plasmids with a miR-125b mimic or inhibitor as follows: Treated, miR-125b mimic (50 nM) co-transfected with the pMir-Sema4D-WT (containing the wild type seed sequence) luciferase reporter; NC, miR-125b inhibitor co-transfected with the pMir-Sema4D-WT luciferase reporter; Blank, miR-125b mimic co-transfected with pMir-Sema4D-MT (containing the mutated seed sequence). Luciferase activities were tested 48 h post-transfection. The data are provided as means ± standard deviation for three replicates from a representative experiment. *** p < 0.001.